Standard

xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice. / Sprimont, Lindsay; Gilloteaux, Jacques; Bouchat, Joanna; Massie, Ann; Nicaise, Charles.

Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018. 2019.

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Harvard

Sprimont, L, Gilloteaux, J, Bouchat, J, Massie, A & Nicaise, C 2019, xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice. in Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018. BELGIAN BRAIN CONGRESS 2018, LUIK, Belgium, 19/10/18. https://doi.org/doi: 10.3389/conf.fnins.2018.95.00035

APA

Sprimont, L., Gilloteaux, J., Bouchat, J., Massie, A., & Nicaise, C. (2019). xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice. In Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018 https://doi.org/doi: 10.3389/conf.fnins.2018.95.00035

Vancouver

Sprimont L, Gilloteaux J, Bouchat J, Massie A, Nicaise C. xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice. In Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018. 2019 https://doi.org/doi: 10.3389/conf.fnins.2018.95.00035

Author

Sprimont, Lindsay ; Gilloteaux, Jacques ; Bouchat, Joanna ; Massie, Ann ; Nicaise, Charles. / xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice. Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018. 2019.

BibTeX

@inbook{4968eeaaec0347279ef9c3be8295eda2,
title = "xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice",
abstract = "xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. The aim of this study is to characterize the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). In situ hybridization allowed to detect xCT mRNA in astrocyte subpopulations and in meningeal fibroblasts in the normal spinal cord. During the early phase of spinal cord injury, xCT mRNA could also be detected in microglial cells and overall mRNA levels were upregulated, peaking at 4 days post-injury. While both injured xCT+/+ and xCT-/- mice recovered partly their motor functions, xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+ cells was unexpectedly much higher in contused xCT-/- than in xCT+/+ spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and influences motor neuron survival.",
keywords = "spinal cord injury, Slc7a11/xCT, system xc-, microglia polarization, astrocyte",
author = "Lindsay Sprimont and Jacques Gilloteaux and Joanna Bouchat and Ann Massie and Charles Nicaise",
year = "2019",
month = "1",
day = "17",
doi = "doi: 10.3389/conf.fnins.2018.95.00035",
language = "English",
booktitle = "Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Li{\`e}ge, 19 october 2018",

}

RIS

TY - CHAP

T1 - xCT/Slc7a11 deletion accelerates motor recovery and improves histological outcomes following cervical spinal contusion in mice

AU - Sprimont, Lindsay

AU - Gilloteaux, Jacques

AU - Bouchat, Joanna

AU - Massie, Ann

AU - Nicaise, Charles

PY - 2019/1/17

Y1 - 2019/1/17

N2 - xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. The aim of this study is to characterize the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). In situ hybridization allowed to detect xCT mRNA in astrocyte subpopulations and in meningeal fibroblasts in the normal spinal cord. During the early phase of spinal cord injury, xCT mRNA could also be detected in microglial cells and overall mRNA levels were upregulated, peaking at 4 days post-injury. While both injured xCT+/+ and xCT-/- mice recovered partly their motor functions, xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+ cells was unexpectedly much higher in contused xCT-/- than in xCT+/+ spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and influences motor neuron survival.

AB - xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. The aim of this study is to characterize the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). In situ hybridization allowed to detect xCT mRNA in astrocyte subpopulations and in meningeal fibroblasts in the normal spinal cord. During the early phase of spinal cord injury, xCT mRNA could also be detected in microglial cells and overall mRNA levels were upregulated, peaking at 4 days post-injury. While both injured xCT+/+ and xCT-/- mice recovered partly their motor functions, xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+ cells was unexpectedly much higher in contused xCT-/- than in xCT+/+ spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and influences motor neuron survival.

KW - spinal cord injury

KW - Slc7a11/xCT

KW - system xc-

KW - microglia polarization

KW - astrocyte

U2 - doi: 10.3389/conf.fnins.2018.95.00035

DO - doi: 10.3389/conf.fnins.2018.95.00035

M3 - Meeting abstract (Book)

BT - Frontiers in Neuroscience, Conference Abstract: Belgian Brain Congress, Liège, 19 october 2018

ER -

ID: 44049099