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Unravelling the Role of System xc- in Epileptogenesis. / Albertini, Giulia; Van Liefferinge, Joeri; Bentea, Eduard-Mihai; Demuyser, Thomas; Merckx, Ellen; Jensen, Cathy; Massie, Ann; Smolders, Ilse Julia.

Center for Neurosciences PhD Day, Brussels, Belgium, march 13, 2014. 2014.

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Harvard

Albertini, G, Van Liefferinge, J, Bentea, E-M, Demuyser, T, Merckx, E, Jensen, C, Massie, A & Smolders, IJ 2014, Unravelling the Role of System xc- in Epileptogenesis. in Center for Neurosciences PhD Day, Brussels, Belgium, march 13, 2014. PhD day Center for Neurosciences, Brussels, Belgium, 13/03/14.

APA

Vancouver

Albertini G, Van Liefferinge J, Bentea E-M, Demuyser T, Merckx E, Jensen C et al. Unravelling the Role of System xc- in Epileptogenesis. In Center for Neurosciences PhD Day, Brussels, Belgium, march 13, 2014. 2014

Author

BibTeX

@inbook{414b2b68db7940dbb0a2b3c3a9a98adb,
title = "Unravelling the Role of System xc- in Epileptogenesis",
abstract = "Unravelling the Role of System xc- in Epileptogenesis G.Albertini Faculty of Medicine & Pharmacy, Center for Neurosciences, Department FASC Vrije Universiteit Brussel Abstract: Epilepsy is a common neurodegenerative disorder which affects about 65 million people worldwide. Approximately, 30{\%} of patients are drug refractory and this data sheds light on the urgent need for innovative antiepileptic therapies to prevent the progression of the disease. In order to find new antiepileptic strategies, two main objectives should be achieved: the identification of new pharmacological targets and the validation of these targets on reliable animal models that mimic the process of epileptogenesis. It is well understood that oxidative stress and excessive glutamatergic activity play a key role in the induction of the neuronal pathology that leads to a variety of neurological diseases, such as epilepsy. The system xc-, a plasma membrane antiporter that imports cystine inside the cell, later to be used for glutathione synthesis, and exports, in turn, glutamate in the extracellular environment, seems to be the conjunction between the attempt of cells to produce antioxidant molecules and increased levels of glutamate, leading potentially to excitotoxicity. Since system xc- is one of the main source of extracellular glutamate in rodent hippocampus and it has been demonstrated that xCT-/- mice are less susceptible to chemoconvulsant drugs, this antiporter became the subject of our interest. The aim of this work is to provide a further investigation on the involvement of system xc- in the generation of seizures and in altering the epileptogenetic process. Therefore we will investigate the hippocampal xCT expression during the various phases of epileptogenesis and we will study the anticonvulsant effects of a knockdown of xCT using Vivo-Morpholino injections. Moreover, we will investigate the seizure susceptibility of both mice overexpressing xCT and xCT-/- mice in the amigdala kindling model and in a post-status epilepticus model. Lastly, since epilepsy is a multifactorial disease, we will investigate the involvement of system xc- in mechanisms of depression.",
keywords = "epilepsy, system xc-, epileptogenesis, glia",
author = "Giulia Albertini and {Van Liefferinge}, Joeri and Eduard-Mihai Bentea and Thomas Demuyser and Ellen Merckx and Cathy Jensen and Ann Massie and Smolders, {Ilse Julia}",
year = "2014",
language = "English",
booktitle = "Center for Neurosciences PhD Day, Brussels, Belgium, march 13, 2014",

}

RIS

TY - CHAP

T1 - Unravelling the Role of System xc- in Epileptogenesis

AU - Albertini, Giulia

AU - Van Liefferinge, Joeri

AU - Bentea, Eduard-Mihai

AU - Demuyser, Thomas

AU - Merckx, Ellen

AU - Jensen, Cathy

AU - Massie, Ann

AU - Smolders, Ilse Julia

PY - 2014

Y1 - 2014

N2 - Unravelling the Role of System xc- in Epileptogenesis G.Albertini Faculty of Medicine & Pharmacy, Center for Neurosciences, Department FASC Vrije Universiteit Brussel Abstract: Epilepsy is a common neurodegenerative disorder which affects about 65 million people worldwide. Approximately, 30% of patients are drug refractory and this data sheds light on the urgent need for innovative antiepileptic therapies to prevent the progression of the disease. In order to find new antiepileptic strategies, two main objectives should be achieved: the identification of new pharmacological targets and the validation of these targets on reliable animal models that mimic the process of epileptogenesis. It is well understood that oxidative stress and excessive glutamatergic activity play a key role in the induction of the neuronal pathology that leads to a variety of neurological diseases, such as epilepsy. The system xc-, a plasma membrane antiporter that imports cystine inside the cell, later to be used for glutathione synthesis, and exports, in turn, glutamate in the extracellular environment, seems to be the conjunction between the attempt of cells to produce antioxidant molecules and increased levels of glutamate, leading potentially to excitotoxicity. Since system xc- is one of the main source of extracellular glutamate in rodent hippocampus and it has been demonstrated that xCT-/- mice are less susceptible to chemoconvulsant drugs, this antiporter became the subject of our interest. The aim of this work is to provide a further investigation on the involvement of system xc- in the generation of seizures and in altering the epileptogenetic process. Therefore we will investigate the hippocampal xCT expression during the various phases of epileptogenesis and we will study the anticonvulsant effects of a knockdown of xCT using Vivo-Morpholino injections. Moreover, we will investigate the seizure susceptibility of both mice overexpressing xCT and xCT-/- mice in the amigdala kindling model and in a post-status epilepticus model. Lastly, since epilepsy is a multifactorial disease, we will investigate the involvement of system xc- in mechanisms of depression.

AB - Unravelling the Role of System xc- in Epileptogenesis G.Albertini Faculty of Medicine & Pharmacy, Center for Neurosciences, Department FASC Vrije Universiteit Brussel Abstract: Epilepsy is a common neurodegenerative disorder which affects about 65 million people worldwide. Approximately, 30% of patients are drug refractory and this data sheds light on the urgent need for innovative antiepileptic therapies to prevent the progression of the disease. In order to find new antiepileptic strategies, two main objectives should be achieved: the identification of new pharmacological targets and the validation of these targets on reliable animal models that mimic the process of epileptogenesis. It is well understood that oxidative stress and excessive glutamatergic activity play a key role in the induction of the neuronal pathology that leads to a variety of neurological diseases, such as epilepsy. The system xc-, a plasma membrane antiporter that imports cystine inside the cell, later to be used for glutathione synthesis, and exports, in turn, glutamate in the extracellular environment, seems to be the conjunction between the attempt of cells to produce antioxidant molecules and increased levels of glutamate, leading potentially to excitotoxicity. Since system xc- is one of the main source of extracellular glutamate in rodent hippocampus and it has been demonstrated that xCT-/- mice are less susceptible to chemoconvulsant drugs, this antiporter became the subject of our interest. The aim of this work is to provide a further investigation on the involvement of system xc- in the generation of seizures and in altering the epileptogenetic process. Therefore we will investigate the hippocampal xCT expression during the various phases of epileptogenesis and we will study the anticonvulsant effects of a knockdown of xCT using Vivo-Morpholino injections. Moreover, we will investigate the seizure susceptibility of both mice overexpressing xCT and xCT-/- mice in the amigdala kindling model and in a post-status epilepticus model. Lastly, since epilepsy is a multifactorial disease, we will investigate the involvement of system xc- in mechanisms of depression.

KW - epilepsy

KW - system xc-

KW - epileptogenesis

KW - glia

M3 - Meeting abstract (Book)

BT - Center for Neurosciences PhD Day, Brussels, Belgium, march 13, 2014

ER -

ID: 2497359