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Tissue distribution of CysAP activity and its relationship to blood pressure and water balance. / Vanderheyden, Patrick; Prieto, Isabelle; Villarejo Villar, Ana Belén; Segarra Robles, Ana Belen; Rosemary, Wangensteen; Immaculada, Banegas; Francisco, Vives; Manuel, Ramirez-Sanchez; De Gasparo, Marc; Stefan, Zorad.

In: Life Sciences, Vol. 134, 26006037, 08.2015, p. 73-78.

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Vanderheyden, P, Prieto, I, Villarejo Villar, AB, Segarra Robles, AB, Rosemary, W, Immaculada, B, Francisco, V, Manuel, R-S, De Gasparo, M & Stefan, Z 2015, 'Tissue distribution of CysAP activity and its relationship to blood pressure and water balance', Life Sciences, vol. 134, 26006037, pp. 73-78. https://doi.org/10.1016/j.lfs.2015.04.023

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Vanderheyden, Patrick ; Prieto, Isabelle ; Villarejo Villar, Ana Belén ; Segarra Robles, Ana Belen ; Rosemary, Wangensteen ; Immaculada, Banegas ; Francisco, Vives ; Manuel, Ramirez-Sanchez ; De Gasparo, Marc ; Stefan, Zorad. / Tissue distribution of CysAP activity and its relationship to blood pressure and water balance. In: Life Sciences. 2015 ; Vol. 134. pp. 73-78.

BibTeX

@article{3b636c51e22249d686fed9ebe35921ea,
title = "Tissue distribution of CysAP activity and its relationship to blood pressure and water balance",
abstract = "AimsTo better understand the functional role of soluble (Sol) and membrane-bound (MB) cystinyl-aminopeptidase (CysAP) activities, we studied differentially their organ distribution in adult male Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) with or without treatment with captopril. We searched for a possible tissue-specific association of CysAP with water balance and blood pressure.Main methodsWe used twenty WKY rats distributed in ten controls and ten captopril-treated, and sixteen SHR divided in eight controls and eight captopril-treated. Captopril (100 mg/kg/day) was administered in drinking water for 4 weeks. Systolic blood pressure, water intake and diuresis were measured individually. CysAP was assayed fluorometrically using l-cystine-di-β-naphthylamide as substrate.Key findingsSol or MB activities were generally higher in SHR compared to WKY notably in hypothalamus and kidney than in the other tissues. Captopril mainly decreased CysAP in SHR whereas it increased in WKY. The distribution of Sol CysAP was more homogeneous among tissues of WKY than SHR. In contrast, the distribution of MB CysAP was more heterogeneous than Sol CysAP in both WKY and SHR. This suggests that MB CysAP activity acts in a more tissue-specific manner than Sol CysAP. The majority of the significant correlations between tissue activities and the measured physiological parameters were observed mostly in renal medulla and hypothalamus.SignificanceSol and MB CysAP activities, acting separately or in concert and mainly in renal medulla, regulate the function of their susceptible endogenous substrates, and may participate meaningfully in the control of blood pressure and fluid balance.",
keywords = "Placental leucine aminopeptidase; Oxytocinase; Vasopressinase; Soluble fraction; Membrane-bound fraction",
author = "Patrick Vanderheyden and Isabelle Prieto and {Villarejo Villar}, {Ana Bel{\'e}n} and {Segarra Robles}, {Ana Belen} and Wangensteen Rosemary and Banegas Immaculada and Vives Francisco and Ramirez-Sanchez Manuel and {De Gasparo}, Marc and Zorad Stefan",
year = "2015",
month = "8",
doi = "10.1016/j.lfs.2015.04.023",
language = "English",
volume = "134",
pages = "73--78",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Tissue distribution of CysAP activity and its relationship to blood pressure and water balance

AU - Vanderheyden, Patrick

AU - Prieto, Isabelle

AU - Villarejo Villar, Ana Belén

AU - Segarra Robles, Ana Belen

AU - Rosemary, Wangensteen

AU - Immaculada, Banegas

AU - Francisco, Vives

AU - Manuel, Ramirez-Sanchez

AU - De Gasparo, Marc

AU - Stefan, Zorad

PY - 2015/8

Y1 - 2015/8

N2 - AimsTo better understand the functional role of soluble (Sol) and membrane-bound (MB) cystinyl-aminopeptidase (CysAP) activities, we studied differentially their organ distribution in adult male Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) with or without treatment with captopril. We searched for a possible tissue-specific association of CysAP with water balance and blood pressure.Main methodsWe used twenty WKY rats distributed in ten controls and ten captopril-treated, and sixteen SHR divided in eight controls and eight captopril-treated. Captopril (100 mg/kg/day) was administered in drinking water for 4 weeks. Systolic blood pressure, water intake and diuresis were measured individually. CysAP was assayed fluorometrically using l-cystine-di-β-naphthylamide as substrate.Key findingsSol or MB activities were generally higher in SHR compared to WKY notably in hypothalamus and kidney than in the other tissues. Captopril mainly decreased CysAP in SHR whereas it increased in WKY. The distribution of Sol CysAP was more homogeneous among tissues of WKY than SHR. In contrast, the distribution of MB CysAP was more heterogeneous than Sol CysAP in both WKY and SHR. This suggests that MB CysAP activity acts in a more tissue-specific manner than Sol CysAP. The majority of the significant correlations between tissue activities and the measured physiological parameters were observed mostly in renal medulla and hypothalamus.SignificanceSol and MB CysAP activities, acting separately or in concert and mainly in renal medulla, regulate the function of their susceptible endogenous substrates, and may participate meaningfully in the control of blood pressure and fluid balance.

AB - AimsTo better understand the functional role of soluble (Sol) and membrane-bound (MB) cystinyl-aminopeptidase (CysAP) activities, we studied differentially their organ distribution in adult male Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) with or without treatment with captopril. We searched for a possible tissue-specific association of CysAP with water balance and blood pressure.Main methodsWe used twenty WKY rats distributed in ten controls and ten captopril-treated, and sixteen SHR divided in eight controls and eight captopril-treated. Captopril (100 mg/kg/day) was administered in drinking water for 4 weeks. Systolic blood pressure, water intake and diuresis were measured individually. CysAP was assayed fluorometrically using l-cystine-di-β-naphthylamide as substrate.Key findingsSol or MB activities were generally higher in SHR compared to WKY notably in hypothalamus and kidney than in the other tissues. Captopril mainly decreased CysAP in SHR whereas it increased in WKY. The distribution of Sol CysAP was more homogeneous among tissues of WKY than SHR. In contrast, the distribution of MB CysAP was more heterogeneous than Sol CysAP in both WKY and SHR. This suggests that MB CysAP activity acts in a more tissue-specific manner than Sol CysAP. The majority of the significant correlations between tissue activities and the measured physiological parameters were observed mostly in renal medulla and hypothalamus.SignificanceSol and MB CysAP activities, acting separately or in concert and mainly in renal medulla, regulate the function of their susceptible endogenous substrates, and may participate meaningfully in the control of blood pressure and fluid balance.

KW - Placental leucine aminopeptidase; Oxytocinase; Vasopressinase; Soluble fraction; Membrane-bound fraction

U2 - 10.1016/j.lfs.2015.04.023

DO - 10.1016/j.lfs.2015.04.023

M3 - Article

VL - 134

SP - 73

EP - 78

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

M1 - 26006037

ER -

ID: 14007695