Current Parkinson's disease research focusses on treatment strategies that interfere with dopaminergic neuronal cell death. The 6-hydroxydopamine (6-OHDA) rat model is ideal for studying the neuroprotective effects of drugs since different degrees of denervation can be established. Injection of 6-OHDA in the medial forebrain bundle (MFB) results in a complete lesion (_90%), whereas administration of this neurotoxin in the striatum creates only a partial lesion (±50%). Although differences can be detected more easily in the MFB lesion model, the neuroprotective action of certain drugs requires the presence of remaining dopaminergic neurons. Therefore, both models are used. In these models we have adapted our methodological approach to obtain maximal data from one animal. After determining motor behavior, we sacrifice the rat, take out the striatum and post-fixate the caudal part of the brain for tyrosine hydroxylase immunostaining. The use of a sucrose-solution with protease inhibitor for homogenization of the striatum allows us to determine the DA content with HPLC as well as the protein content (GDNF, GAP43,...) in the same sample. Using this approach we are currently investigating the neuroprotective effects of different ligands of the renin-angiontensin system.
Original languageEnglish
Title of host publication7th bi-annual meeting of the Belgian Society for Neuroscience
Publication statusPublished - 2008

Publication series

Name7th bi-annual meeting of the Belgian Society for Neuroscience

    Research areas

  • neuroprotection, 6-hydroxydopamine, Parkinson's disease

ID: 1751650