• Sigrid Ottestad Hansen
  • You Zhou
  • V. Follin-Arbelet
  • Hideyo SATO
  • Ann Massie
  • Niels C Danbolt
The cysteine/glutamate exchanger system xc-, with Xct (slc7a11) as specific subunit, allows cells to take up cystine by releasing glutamate. Consequently, system xc promotes glutathione synthesis which depends on cysteine (reduced cystine), and, in doing that, system xc also contributes to increases in extracellular glutamate. In fact, in several neurological disorders system xc- is believed to be responsible for releasing excessive amounts of glutamate as loss of system xc- (xCT knockout mice) has been shown to be protective in many disease models To better understand the roles of system xc- in the normal and diseased brain it is important to determine the exact localizations and expression levels. This, however, has been remarkably hard as many of the antibodies available do not react specifically with xCT. Recently, we have produced specific xCT antibodies (Van Liefferinge et al., 2016). Here, we have tested them further using xCT knockout mice as negative controls. We obtain consistent labeling on brain sections and on Western blots. With these we are now determining the exact cellular localization of xCT using confocal and superresolution microscopy in combination with antibodies to various marker proteins (e.g. EAAT1,IBA1, CD31, NeuN and others).Van Liefferinge J, Bentea E, Demuyser T, Albertini G, Follin-Arbelet V, Holmseth S, Merckx E, Sato H, Aerts JL, Smolders I, Arckens L, Danbolt NC, Massie A (2016) Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed. J Comp Neurol. 524:1015-32. Abstract : Index 720
Original languageEnglish
Publication statusPublished - 2016
Event10th FENS Forum of Neuroscience - Copenhagen, Denmark
Duration: 2 Jul 20166 Jul 2016

Conference

Conference10th FENS Forum of Neuroscience
CountryDenmark
CityCopenhagen
Period2/07/166/07/16

    Research areas

  • xCT, glutamate-cystine

ID: 28272245