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The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle. / Gelkop, S.; Sobarzo, A.; Brangel, P. ; Vincke, Cecile; Estevens Romão, Ema; Fedida-Metula, S.; Strom, N.; Ataliba, I.; Mwiine, F.N.; Ochwo, S.; Velazquez-Salinas, L.; Mc Kendry, R.A.; Muyldermans, Serge; Lutwama, J.J.; Rieder, E.; Yavelsky, V.; Lobel, Leslie.

In: Frontiers in Veterinary Science, Vol. 5, No. OCT, 250, 12.10.2018, p. 250.

Research output: Contribution to journalArticleResearchpeer-review

Harvard

Gelkop, S, Sobarzo, A, Brangel, P, Vincke, C, Estevens Romão, E, Fedida-Metula, S, Strom, N, Ataliba, I, Mwiine, FN, Ochwo, S, Velazquez-Salinas, L, Mc Kendry, RA, Muyldermans, S, Lutwama, JJ, Rieder, E, Yavelsky, V & Lobel, L 2018, 'The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle' Frontiers in Veterinary Science, vol. 5, no. OCT, 250, pp. 250. https://doi.org/10.3389/fvets.2018.00250

APA

Vancouver

Author

Gelkop, S. ; Sobarzo, A. ; Brangel, P. ; Vincke, Cecile ; Estevens Romão, Ema ; Fedida-Metula, S. ; Strom, N. ; Ataliba, I. ; Mwiine, F.N. ; Ochwo, S. ; Velazquez-Salinas, L. ; Mc Kendry, R.A. ; Muyldermans, Serge ; Lutwama, J.J. ; Rieder, E. ; Yavelsky, V. ; Lobel, Leslie. / The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle. In: Frontiers in Veterinary Science. 2018 ; Vol. 5, No. OCT. pp. 250.

BibTeX

@article{8d61e2bae6cf47fe8876306e7ced2040,
title = "The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle",
abstract = "Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected and vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these tests vary in their sensitivity and specificity and are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, have demonstrated great efficacy for the development of serological diagnostics. This study describes the development of a novel Nanobody-based FMD 3ABC competitive ELISA, for the serological detection of antibodies against FMD Non-Structural Proteins (NSP) in Uganda cattle herds. This in-house ELISA was validated using more than 600 sera from different Uganda districts, and virus serotype specificities. The evaluation of the performance of the assay demonstrated high diagnostic sensitivity and specificity of 94 {\%} (95 {\%} CI: 88.9-97.2), and 97.67 {\%} (95 {\%} CI: 94.15-99.36) respectively, as well as the capability to detect NSP-specific antibodies against multiple FMD serotype infections. In comparison with the commercial PrioCHECK FMDV NSP-FMD test, there was a strong concordance and high correlation and agreement in the performance of the two tests. This new developed Nanobody based FMD 3ABC competitive ELISA could clearly benefit routine disease diagnosis, the establishment of disease-free zones, and the improvement of FMD management and control in endemically complex environments, such as those found in Africa.",
keywords = "Antibodies, ELISA, Foot and mouth disease, Nanobodies, Non-structural proteins",
author = "S. Gelkop and A. Sobarzo and P. Brangel and Cecile Vincke and {Estevens Rom{\~a}o}, Ema and S. Fedida-Metula and N. Strom and I. Ataliba and F.N. Mwiine and S. Ochwo and L. Velazquez-Salinas and {Mc Kendry}, R.A. and Serge Muyldermans and J.J. Lutwama and E. Rieder and V. Yavelsky and Leslie Lobel",
year = "2018",
month = "10",
day = "12",
doi = "10.3389/fvets.2018.00250",
language = "English",
volume = "5",
pages = "250",
journal = "Frontiers in Veterinary Science",
issn = "22971769",
number = "OCT",

}

RIS

TY - JOUR

T1 - The development and validation of a novel Nanobody-Based competitive ELISA for the detection of foot and mouth disease 3ABC antibodies in cattle

AU - Gelkop, S.

AU - Sobarzo, A.

AU - Brangel, P.

AU - Vincke, Cecile

AU - Estevens Romão, Ema

AU - Fedida-Metula, S.

AU - Strom, N.

AU - Ataliba, I.

AU - Mwiine, F.N.

AU - Ochwo, S.

AU - Velazquez-Salinas, L.

AU - Mc Kendry, R.A.

AU - Muyldermans, Serge

AU - Lutwama, J.J.

AU - Rieder, E.

AU - Yavelsky, V.

AU - Lobel, Leslie

PY - 2018/10/12

Y1 - 2018/10/12

N2 - Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected and vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these tests vary in their sensitivity and specificity and are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, have demonstrated great efficacy for the development of serological diagnostics. This study describes the development of a novel Nanobody-based FMD 3ABC competitive ELISA, for the serological detection of antibodies against FMD Non-Structural Proteins (NSP) in Uganda cattle herds. This in-house ELISA was validated using more than 600 sera from different Uganda districts, and virus serotype specificities. The evaluation of the performance of the assay demonstrated high diagnostic sensitivity and specificity of 94 % (95 % CI: 88.9-97.2), and 97.67 % (95 % CI: 94.15-99.36) respectively, as well as the capability to detect NSP-specific antibodies against multiple FMD serotype infections. In comparison with the commercial PrioCHECK FMDV NSP-FMD test, there was a strong concordance and high correlation and agreement in the performance of the two tests. This new developed Nanobody based FMD 3ABC competitive ELISA could clearly benefit routine disease diagnosis, the establishment of disease-free zones, and the improvement of FMD management and control in endemically complex environments, such as those found in Africa.

AB - Effective management of foot and mouth disease (FMD) requires diagnostic tests to distinguish between infected and vaccinated animals (DIVA). To address this need, several enzyme-linked immunosorbent assay (ELISA) platforms have been developed, however, these tests vary in their sensitivity and specificity and are very expensive for developing countries. Camelid-derived single-domain antibodies fragments so-called Nanobodies, have demonstrated great efficacy for the development of serological diagnostics. This study describes the development of a novel Nanobody-based FMD 3ABC competitive ELISA, for the serological detection of antibodies against FMD Non-Structural Proteins (NSP) in Uganda cattle herds. This in-house ELISA was validated using more than 600 sera from different Uganda districts, and virus serotype specificities. The evaluation of the performance of the assay demonstrated high diagnostic sensitivity and specificity of 94 % (95 % CI: 88.9-97.2), and 97.67 % (95 % CI: 94.15-99.36) respectively, as well as the capability to detect NSP-specific antibodies against multiple FMD serotype infections. In comparison with the commercial PrioCHECK FMDV NSP-FMD test, there was a strong concordance and high correlation and agreement in the performance of the two tests. This new developed Nanobody based FMD 3ABC competitive ELISA could clearly benefit routine disease diagnosis, the establishment of disease-free zones, and the improvement of FMD management and control in endemically complex environments, such as those found in Africa.

KW - Antibodies

KW - ELISA

KW - Foot and mouth disease

KW - Nanobodies

KW - Non-structural proteins

UR - http://www.scopus.com/inward/record.url?scp=85055805051&partnerID=8YFLogxK

U2 - 10.3389/fvets.2018.00250

DO - 10.3389/fvets.2018.00250

M3 - Article

VL - 5

SP - 250

JO - Frontiers in Veterinary Science

JF - Frontiers in Veterinary Science

SN - 22971769

IS - OCT

M1 - 250

ER -

ID: 45492415