The preparation of a highly efficient chiral liquid chromatography (LC) column is explored by dynamically coating a reversed-phase porous silicon pillar array column with hydroxypropyl-beta-cyclodextrin (Hp-beta-CD) as the chiral selector. Analyte mixtures composed of non-steroidal anti-inflammatory drugs were tested to reveal the enantioseparation potential of the column. The mechanism of chiral discrimination was investigated. The adsorbed Hp-beta-CDs on the column surface experience different interaction with enantiomers. The chiral stationary phase showed satisfying stability and could be easily restored by recovering the selector with sufficient flushing and repeating the loading procedure. The peak capacity of the column was evaluated, and it was found high enough to separate three enantiomer couples using a one-dimensional LC technique. (C) 2019 Elsevier B.V. All rights reserved.
Original languageEnglish
Article number460752
JournalJournal of Chromatography A
Publication statusPublished - 29 Mar 2020

    Research areas

  • Chiral, Dynamic coating, One-dimensional liquid chromatography, Non-steroidal anti-inflammatory drugs, Porous silicon pillar array column

ID: 51957392