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Quantification of piritramide in human colostrum. / Van Eeckhaut, A; Van Schoors, J; Van Wanseele, Y; Poelaert, J; Michotte, Y; Vanfleteren, Patsy.

In: Journal of Clinical Pharmacy and Therapeutics, Vol. 42, No. 3, 06.2017, p. 306-310.

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@article{b05c253661944505911964e938111a74,
title = "Quantification of piritramide in human colostrum",
abstract = "WHAT IS KNOWN AND OBJECTIVE: In our university hospital (UZBrussel), one of the options to control post-operative pain after a Caesarean section under general anaesthesia is to administer piritramide by patient-controlled intravenous analgesia (PCIA). As no information is available about the possible transfer of this synthetic narcotic analgesic into breastmilk, women are frequently advised not to breastfeed their newborn. A sensitive liquid chromatographic (LC) method coupled with UV detection will therefore be developed and validated for the quantification of piritramide in colostrum samples to evaluate the presence of the analgesic in the first milk.METHODS: The method included the isolation and concentration of piritramide from colostrum using protein precipitation and solid-phase extraction (SPE) using a mixed-mode cation exchange sorbent. Subsequently, the extracted samples were analysed on a microbore C18 column (1 mm id) and a mobile phase consisting of 15 mm ammonium hydroxide in methanol/tetrahydrofuran/water 50 : 10 : 40 V/V/V.RESULTS AND DISCUSSION: As colostrum contains a high amount of proteins, mixed-mode cation exchange SPE was preceded by a 1 : 2 dilution and protein precipitation with phosphoric acid followed by double centrifugation of the samples. The reversed-phase LC-UV method used a mobile phase at alkaline pH to obtain a selective method for piritramide and the internal standard pipamperone. After investigating the validation characteristics (linearity, accuracy, precision and stability), samples from ten patients who had received piritramide via PCIA during the first 48 h post-partum were analysed.WHAT IS NEW AND CONCLUSION: To the best of our knowledge, this is the first method described for the quantification of the synthetic narcotic analgesic piritramide in colostrum samples. The obtained results suggest that after the administration of this opioid by PCIA to nursing mothers low concentrations of piritramide can be found in the first milk, but are mostly below the limit of quantification of 30 ng/mL.",
keywords = "Analgesia, Patient-Controlled, Analgesics, Opioid, Chromatography, Liquid, Chromatography, Reverse-Phase, Colostrum, Female, Humans, Pirinitramide, Reproducibility of Results, Spectrophotometry, Ultraviolet, Journal Article, Validation Studies",
author = "{Van Eeckhaut}, A and {Van Schoors}, J and {Van Wanseele}, Y and J Poelaert and Y Michotte and Patsy Vanfleteren",
note = "{\circledC} 2017 John Wiley & Sons Ltd.",
year = "2017",
month = "6",
doi = "10.1111/jcpt.12515",
language = "English",
volume = "42",
pages = "306--310",
journal = "Journal of Clinical Pharmacy and Therapeutics",
issn = "0269-4727",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Quantification of piritramide in human colostrum

AU - Van Eeckhaut, A

AU - Van Schoors, J

AU - Van Wanseele, Y

AU - Poelaert, J

AU - Michotte, Y

AU - Vanfleteren, Patsy

N1 - © 2017 John Wiley & Sons Ltd.

PY - 2017/6

Y1 - 2017/6

N2 - WHAT IS KNOWN AND OBJECTIVE: In our university hospital (UZBrussel), one of the options to control post-operative pain after a Caesarean section under general anaesthesia is to administer piritramide by patient-controlled intravenous analgesia (PCIA). As no information is available about the possible transfer of this synthetic narcotic analgesic into breastmilk, women are frequently advised not to breastfeed their newborn. A sensitive liquid chromatographic (LC) method coupled with UV detection will therefore be developed and validated for the quantification of piritramide in colostrum samples to evaluate the presence of the analgesic in the first milk.METHODS: The method included the isolation and concentration of piritramide from colostrum using protein precipitation and solid-phase extraction (SPE) using a mixed-mode cation exchange sorbent. Subsequently, the extracted samples were analysed on a microbore C18 column (1 mm id) and a mobile phase consisting of 15 mm ammonium hydroxide in methanol/tetrahydrofuran/water 50 : 10 : 40 V/V/V.RESULTS AND DISCUSSION: As colostrum contains a high amount of proteins, mixed-mode cation exchange SPE was preceded by a 1 : 2 dilution and protein precipitation with phosphoric acid followed by double centrifugation of the samples. The reversed-phase LC-UV method used a mobile phase at alkaline pH to obtain a selective method for piritramide and the internal standard pipamperone. After investigating the validation characteristics (linearity, accuracy, precision and stability), samples from ten patients who had received piritramide via PCIA during the first 48 h post-partum were analysed.WHAT IS NEW AND CONCLUSION: To the best of our knowledge, this is the first method described for the quantification of the synthetic narcotic analgesic piritramide in colostrum samples. The obtained results suggest that after the administration of this opioid by PCIA to nursing mothers low concentrations of piritramide can be found in the first milk, but are mostly below the limit of quantification of 30 ng/mL.

AB - WHAT IS KNOWN AND OBJECTIVE: In our university hospital (UZBrussel), one of the options to control post-operative pain after a Caesarean section under general anaesthesia is to administer piritramide by patient-controlled intravenous analgesia (PCIA). As no information is available about the possible transfer of this synthetic narcotic analgesic into breastmilk, women are frequently advised not to breastfeed their newborn. A sensitive liquid chromatographic (LC) method coupled with UV detection will therefore be developed and validated for the quantification of piritramide in colostrum samples to evaluate the presence of the analgesic in the first milk.METHODS: The method included the isolation and concentration of piritramide from colostrum using protein precipitation and solid-phase extraction (SPE) using a mixed-mode cation exchange sorbent. Subsequently, the extracted samples were analysed on a microbore C18 column (1 mm id) and a mobile phase consisting of 15 mm ammonium hydroxide in methanol/tetrahydrofuran/water 50 : 10 : 40 V/V/V.RESULTS AND DISCUSSION: As colostrum contains a high amount of proteins, mixed-mode cation exchange SPE was preceded by a 1 : 2 dilution and protein precipitation with phosphoric acid followed by double centrifugation of the samples. The reversed-phase LC-UV method used a mobile phase at alkaline pH to obtain a selective method for piritramide and the internal standard pipamperone. After investigating the validation characteristics (linearity, accuracy, precision and stability), samples from ten patients who had received piritramide via PCIA during the first 48 h post-partum were analysed.WHAT IS NEW AND CONCLUSION: To the best of our knowledge, this is the first method described for the quantification of the synthetic narcotic analgesic piritramide in colostrum samples. The obtained results suggest that after the administration of this opioid by PCIA to nursing mothers low concentrations of piritramide can be found in the first milk, but are mostly below the limit of quantification of 30 ng/mL.

KW - Analgesia, Patient-Controlled

KW - Analgesics, Opioid

KW - Chromatography, Liquid

KW - Chromatography, Reverse-Phase

KW - Colostrum

KW - Female

KW - Humans

KW - Pirinitramide

KW - Reproducibility of Results

KW - Spectrophotometry, Ultraviolet

KW - Journal Article

KW - Validation Studies

U2 - 10.1111/jcpt.12515

DO - 10.1111/jcpt.12515

M3 - Article

C2 - 28295465

VL - 42

SP - 306

EP - 310

JO - Journal of Clinical Pharmacy and Therapeutics

JF - Journal of Clinical Pharmacy and Therapeutics

SN - 0269-4727

IS - 3

ER -

ID: 36171352