The graTA operon from Pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in solution and crystallizes in space group P2 1, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 Å, β = 92.6°. The crystals are likely to contain two GraA dimers in the asymmetric unit and diffract to 1.9 Å resolution. The GraTA complex forms a heterotetramer in solution. Crystals of the GraTA complex diffracted to 2.2 Å resolution and are most likely to contain a single heterotetrameric GraTA complex in the asymmetric unit. They belong to space group P4 1 or P4 3, with unit-cell parameters a = b = 56.0, c = 128.2 Å. The GraA-operator complex consists of a 33 bp operator region that binds two GraA dimers. It crystallizes in space group P3 1 or P3 2, with unit-cell parameters a = b = 105.6, c = 149.9 Å. These crystals diffract to 3.8 Å resolution.The antitoxin GraA from P. putida and its complexes with the toxin GraT and with the 33 bp operator of the graTA operon were crystallized.

Original languageEnglish
Pages (from-to)455-462
Number of pages8
JournalActa Crystallographica Section F - Structural Biology Communications
VolumeF73
Issue number8
DOIs
Publication statusPublished - Aug 2017

    Research areas

  • Amino Acid Sequence, Antitoxins/chemistry, Bacterial Proteins/chemistry, Bacterial Toxins/chemistry, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Escherichia coli/genetics, Gene Expression, Gene Expression Regulation, Bacterial, Genetic Vectors/chemistry, Operon, Protein Binding, Protein Multimerization, Pseudomonas putida/chemistry, Recombinant Fusion Proteins/chemistry, X-Ray Diffraction

ID: 32659507