DOI

The present paper describes the application of a sol-gel procedure on radially elongated pillars (REPs) using tetramethoxysilane and methyltrimethoxysilane. After octadecylsilylation, the quality of the porous layered REP (PLREP) columns was evaluated by in-situ determination of migration velocities and band broadening of coumarin dyes with fluorescence microscopy in reversed-phase liquid chromatography. Based on the increase in retention due to the sol-gel process, an increase in accessible specific surface by a factor of 112 was observed. Argon physisorption measurements on bulk monoliths prepared with the same method revealed a predominant pore size of 91 angstrom. Plate heights as low as 0.4-0.8 mu m (k=0-1.97) could be obtained thanks to the very low dispersion of the REP format and to the fact that the applied silica layer was conformally and uniformly deposited on the flow-through channels. A kinetic plot analysis demonstrated that the studied PLREP column will deliver more theoretical plates per unit of time than a 5 m core shell packed bed when more than 1.0 x 10(4) theoretical plates are required. (C) 2017 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)234-241
Number of pages8
JournalJ. Chromatogr. A
Volume1523
DOIs
StatePublished - 10 Nov 2017

    Research areas

  • LIQUID-CHROMATOGRAPHY;, ARRAY COLUMNS, CAPILLARY ELECTROCHROMATOGRAPHY, KINETIC PERFORMANCE, STATIONARY-PHASE, SHELL PARTICLES, LOW-DISPERSION, NEW-GENERATION, B-TERM;, Separation Methods

ID: 35723297