DOI

  • Sara Vieira-Silva
  • Jun Wang
  • Alexander Kurilshikov
  • Marc Jan Bonder
  • Mireia Valles-Colomer
  • Raul Y Tito
  • Chloë Verspecht
  • Daniel Homola
  • Roberto Garcia
  • Ettje F Tigchelaar
  • Jingyuan Fu
  • Liesbet Henckaerts
  • Alexandra Zhernakova
  • Cisca Wijmenga

Fecal microbiome variation in the average, healthy population has remained under-investigated. Here, we analyzed two independent, extensively phenotyped cohorts: the Belgian Flemish Gut Flora Project (FGFP; discovery cohort; N = 1106) and the Dutch LifeLines-DEEP study (LLDeep; replication; N = 1135). Integration with global data sets (N combined = 3948) revealed a 14-genera core microbiota, but the 664 identified genera still underexplore total gut diversity. Sixty-nine clinical and questionnaire-based covariates were found associated to microbiota compositional variation with a 92% replication rate. Stool consistency showed the largest effect size, whereas medication explained largest total variance and interacted with other covariate-microbiota associations. Early-life events such as birth mode were not reflected in adult microbiota composition. Finally, we found that proposed disease marker genera associated to host covariates, urging inclusion of the latter in study design.

Original languageEnglish
Pages (from-to)560-564
Number of pages5
JournalScience
Volume352
Issue number6285
DOIs
Publication statusPublished - 29 Apr 2016

    Research areas

  • Bacteria, Belgium, Cohort Studies, Drug Interactions, Feces, Gastrointestinal Microbiome, Humans

ID: 25511531