Standard

New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization. / Rakosy-Tican, Elena; Loerincz-Besenyei, Enikoe; Molnar, Imola; Thieme, Ramona; Hartung, Frank; Sprink, Thorben; Antonova, Olga; Famelaer, Ivan; Angenon, Geert; Aurori, Adriana.

In: Frontiers in Plant Science, Vol. 10, 3, 22.01.2019.

Research output: Contribution to journalArticle

Harvard

Rakosy-Tican, E, Loerincz-Besenyei, E, Molnar, I, Thieme, R, Hartung, F, Sprink, T, Antonova, O, Famelaer, I, Angenon, G & Aurori, A 2019, 'New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization', Frontiers in Plant Science, vol. 10, 3. https://doi.org/10.3389/fpls.2019.00003

APA

Rakosy-Tican, E., Loerincz-Besenyei, E., Molnar, I., Thieme, R., Hartung, F., Sprink, T., ... Aurori, A. (2019). New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization. Frontiers in Plant Science, 10, [3]. https://doi.org/10.3389/fpls.2019.00003

Vancouver

Rakosy-Tican E, Loerincz-Besenyei E, Molnar I, Thieme R, Hartung F, Sprink T et al. New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization. Frontiers in Plant Science. 2019 Jan 22;10. 3. https://doi.org/10.3389/fpls.2019.00003

Author

Rakosy-Tican, Elena ; Loerincz-Besenyei, Enikoe ; Molnar, Imola ; Thieme, Ramona ; Hartung, Frank ; Sprink, Thorben ; Antonova, Olga ; Famelaer, Ivan ; Angenon, Geert ; Aurori, Adriana. / New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization. In: Frontiers in Plant Science. 2019 ; Vol. 10.

BibTeX

@article{d5aa6204a8cb4092a77ce41826bfd247,
title = "New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization",
abstract = "As plants are sessile they need a very efficient system for repairing damage doneby external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘D{\'e}sir{\'e}e’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; thesemutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatichybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.",
keywords = "Antisense strategy, AtMSH2 gene, Dominant negative mutant, Microsatellite instability, Mismatch repair deficiency, Solanum chacoense Bitt, ‘mutator’ phenotype",
author = "Elena Rakosy-Tican and Enikoe Loerincz-Besenyei and Imola Molnar and Ramona Thieme and Frank Hartung and Thorben Sprink and Olga Antonova and Ivan Famelaer and Geert Angenon and Adriana Aurori",
year = "2019",
month = "1",
day = "22",
doi = "10.3389/fpls.2019.00003",
language = "English",
volume = "10",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization

AU - Rakosy-Tican, Elena

AU - Loerincz-Besenyei, Enikoe

AU - Molnar, Imola

AU - Thieme, Ramona

AU - Hartung, Frank

AU - Sprink, Thorben

AU - Antonova, Olga

AU - Famelaer, Ivan

AU - Angenon, Geert

AU - Aurori, Adriana

PY - 2019/1/22

Y1 - 2019/1/22

N2 - As plants are sessile they need a very efficient system for repairing damage doneby external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘Désirée’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; thesemutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatichybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.

AB - As plants are sessile they need a very efficient system for repairing damage doneby external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘Désirée’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; thesemutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatichybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.

KW - Antisense strategy

KW - AtMSH2 gene

KW - Dominant negative mutant

KW - Microsatellite instability

KW - Mismatch repair deficiency

KW - Solanum chacoense Bitt

KW - ‘mutator’ phenotype

UR - http://www.scopus.com/inward/record.url?scp=85062565955&partnerID=8YFLogxK

U2 - 10.3389/fpls.2019.00003

DO - 10.3389/fpls.2019.00003

M3 - Article

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 3

ER -

ID: 44030439