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Nanobody-based chromatin immunoprecipitation. / NGUYEN DUC, Trong; Hassanzadeh Ghassabeh, Gholamreza; Saerens, Dirk; Peeters, Eveline; Charlier, Daniel; Muyldermans, Serge.

Single Domain Antibodies: Methods an Protocols. ed. / Dirk Saerens; Serge Muyldermans. Humana Press, 2012. p. 491-505 (Methods in Molecular Biology; Vol. 911).

Research output: Chapter in Book/Report/Conference proceedingChapter

Harvard

NGUYEN DUC, T, Hassanzadeh Ghassabeh, G, Saerens, D, Peeters, E, Charlier, D & Muyldermans, S 2012, Nanobody-based chromatin immunoprecipitation. in D Saerens & S Muyldermans (eds), Single Domain Antibodies: Methods an Protocols. Methods in Molecular Biology, vol. 911, Humana Press, pp. 491-505.

APA

NGUYEN DUC, T., Hassanzadeh Ghassabeh, G., Saerens, D., Peeters, E., Charlier, D., & Muyldermans, S. (2012). Nanobody-based chromatin immunoprecipitation. In D. Saerens, & S. Muyldermans (Eds.), Single Domain Antibodies: Methods an Protocols (pp. 491-505). (Methods in Molecular Biology; Vol. 911). Humana Press.

Vancouver

NGUYEN DUC T, Hassanzadeh Ghassabeh G, Saerens D, Peeters E, Charlier D, Muyldermans S. Nanobody-based chromatin immunoprecipitation. In Saerens D, Muyldermans S, editors, Single Domain Antibodies: Methods an Protocols. Humana Press. 2012. p. 491-505. (Methods in Molecular Biology).

Author

NGUYEN DUC, Trong ; Hassanzadeh Ghassabeh, Gholamreza ; Saerens, Dirk ; Peeters, Eveline ; Charlier, Daniel ; Muyldermans, Serge. / Nanobody-based chromatin immunoprecipitation. Single Domain Antibodies: Methods an Protocols. editor / Dirk Saerens ; Serge Muyldermans. Humana Press, 2012. pp. 491-505 (Methods in Molecular Biology).

BibTeX

@inbook{4bbd44d783d844b78bfa0c71bb9db6b0,
title = "Nanobody-based chromatin immunoprecipitation",
abstract = "Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale. The success of ChIP largely depends on the quality of antibodies. Although polyclonal antibodies have been successfully used for ChIP, their production requires regular immunization and they exhibit high aspecificity and batch to batch variability. These problems can be circumvented by generating monoclonal antibodies (mAbs) via hybridoma technology. However, such mAbs do not often capture DNA-protein complexes and are not amenable to engineering. Nanobodies are recombinant single domain antibody fragments derived from camelid Heavy-Chain antibodies. Nanobodies exhibit high affinity and specificity towards their cognate antigens and often capture their target antigens in solution. Moreover, the Nanobody genes can be easily tailored to streamline ChIP. Here, we describe a Nanobody-based ChIP protocol which we have successfully used for genome-wide identification of the binding sites of the low-abundant transcription factor Ss-LrpB from the hyperthermoacidophilic archaeon Sulfolobus solfataricus.",
keywords = "Chromatin immunoprecipitation, DNA-protein interaction, Nanobody, Single domain antibody, Sulfolobus solfataricus",
author = "{NGUYEN DUC}, Trong and {Hassanzadeh Ghassabeh}, Gholamreza and Dirk Saerens and Eveline Peeters and Daniel Charlier and Serge Muyldermans",
note = "John M. Walker",
year = "2012",
month = "7",
day = "18",
language = "English",
isbn = "978-1-61779-967-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "491--505",
editor = "Dirk Saerens and Serge Muyldermans",
booktitle = "Single Domain Antibodies",
address = "United States",

}

RIS

TY - CHAP

T1 - Nanobody-based chromatin immunoprecipitation

AU - NGUYEN DUC, Trong

AU - Hassanzadeh Ghassabeh, Gholamreza

AU - Saerens, Dirk

AU - Peeters, Eveline

AU - Charlier, Daniel

AU - Muyldermans, Serge

N1 - John M. Walker

PY - 2012/7/18

Y1 - 2012/7/18

N2 - Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale. The success of ChIP largely depends on the quality of antibodies. Although polyclonal antibodies have been successfully used for ChIP, their production requires regular immunization and they exhibit high aspecificity and batch to batch variability. These problems can be circumvented by generating monoclonal antibodies (mAbs) via hybridoma technology. However, such mAbs do not often capture DNA-protein complexes and are not amenable to engineering. Nanobodies are recombinant single domain antibody fragments derived from camelid Heavy-Chain antibodies. Nanobodies exhibit high affinity and specificity towards their cognate antigens and often capture their target antigens in solution. Moreover, the Nanobody genes can be easily tailored to streamline ChIP. Here, we describe a Nanobody-based ChIP protocol which we have successfully used for genome-wide identification of the binding sites of the low-abundant transcription factor Ss-LrpB from the hyperthermoacidophilic archaeon Sulfolobus solfataricus.

AB - Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale. The success of ChIP largely depends on the quality of antibodies. Although polyclonal antibodies have been successfully used for ChIP, their production requires regular immunization and they exhibit high aspecificity and batch to batch variability. These problems can be circumvented by generating monoclonal antibodies (mAbs) via hybridoma technology. However, such mAbs do not often capture DNA-protein complexes and are not amenable to engineering. Nanobodies are recombinant single domain antibody fragments derived from camelid Heavy-Chain antibodies. Nanobodies exhibit high affinity and specificity towards their cognate antigens and often capture their target antigens in solution. Moreover, the Nanobody genes can be easily tailored to streamline ChIP. Here, we describe a Nanobody-based ChIP protocol which we have successfully used for genome-wide identification of the binding sites of the low-abundant transcription factor Ss-LrpB from the hyperthermoacidophilic archaeon Sulfolobus solfataricus.

KW - Chromatin immunoprecipitation

KW - DNA-protein interaction

KW - Nanobody

KW - Single domain antibody

KW - Sulfolobus solfataricus

M3 - Chapter

SN - 978-1-61779-967-9

T3 - Methods in Molecular Biology

SP - 491

EP - 505

BT - Single Domain Antibodies

A2 - Saerens, Dirk

A2 - Muyldermans, Serge

PB - Humana Press

ER -

ID: 2208519