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Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors. / Van der Perren, Anke; Toelen, Jaan; Casteels, Cindy; Macchi, Francesca; Van Rompuy, Anne-Sophie; Sarre, Sophie; Casadei, Nicolas; Nuber, Silke; Himmelreich, Uwe; Osorio Garcia, Maria Isabel ; Michotte, Yvette; D'Hooge, Rudi; Bormans, Guy; Van Laere, Koen; Gijsbers, Rik; Van den Haute, Chris; Debyser, Zeger; Baekelandt, Veerle.

In: Neurobiology of Aging, Vol. 36, 2015, p. 1543-1558.

Research output: Contribution to journalArticle

Harvard

Van der Perren, A, Toelen, J, Casteels, C, Macchi, F, Van Rompuy, A-S, Sarre, S, Casadei, N, Nuber, S, Himmelreich, U, Osorio Garcia, MI, Michotte, Y, D'Hooge, R, Bormans, G, Van Laere, K, Gijsbers, R, Van den Haute, C, Debyser, Z & Baekelandt, V 2015, 'Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors.', Neurobiology of Aging, vol. 36, pp. 1543-1558.

APA

Van der Perren, A., Toelen, J., Casteels, C., Macchi, F., Van Rompuy, A-S., Sarre, S., ... Baekelandt, V. (2015). Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors. Neurobiology of Aging, 36, 1543-1558.

Vancouver

Author

Van der Perren, Anke ; Toelen, Jaan ; Casteels, Cindy ; Macchi, Francesca ; Van Rompuy, Anne-Sophie ; Sarre, Sophie ; Casadei, Nicolas ; Nuber, Silke ; Himmelreich, Uwe ; Osorio Garcia, Maria Isabel ; Michotte, Yvette ; D'Hooge, Rudi ; Bormans, Guy ; Van Laere, Koen ; Gijsbers, Rik ; Van den Haute, Chris ; Debyser, Zeger ; Baekelandt, Veerle. / Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors. In: Neurobiology of Aging. 2015 ; Vol. 36. pp. 1543-1558.

BibTeX

@article{8a4ddee99f6347d395c9b4685da9d2ec,
title = "Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors.",
abstract = "Testing of new therapeutic strategies for Parkinson's disease (PD) is currently hampered by the lack of relevant and reproducible animal models. Here, we developed a robust rat model for PD by injection of adeno-associated viral vectors (rAAV2/7) encoding α-synuclein into the substantia nigra, resulting in reproducible nigrostriatal pathology and behavioral deficits in a 4-week time period. Progressive dopaminergic dysfunction was corroborated by histopathologic and biochemical analysis, motor behavior testing and in vivo microdialysis. L-DOPA treatment was found to reverse the behavioral phenotype. Non-invasive positron emission tomography imaging and magnetic resonance spectroscopy allowed longitudinal monitoring of neurodegeneration. In addition, insoluble α-synuclein aggregates were formed in this model. This α-synuclein rat model shows improved face and predictive validity, and therefore offers the possibility to reliably test novel therapeutics. Furthermore, it will be of great value for further research into the molecular pathogenesis of PD and the importance of α-synuclein aggregation in the disease process.",
keywords = "Adeno-associated viral vectors",
author = "{Van der Perren}, Anke and Jaan Toelen and Cindy Casteels and Francesca Macchi and {Van Rompuy}, Anne-Sophie and Sophie Sarre and Nicolas Casadei and Silke Nuber and Uwe Himmelreich and {Osorio Garcia}, {Maria Isabel} and Yvette Michotte and Rudi D'Hooge and Guy Bormans and {Van Laere}, Koen and Rik Gijsbers and {Van den Haute}, Chris and Zeger Debyser and Veerle Baekelandt",
year = "2015",
language = "English",
volume = "36",
pages = "1543--1558",
journal = "Neurobiology of Aging",
issn = "0197-4580",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Longitudinal follow-up and characterization of a robust rat model for Parkinson's disease based on overexpression of alpha-synuclein with adeno-associated viral vectors.

AU - Van der Perren, Anke

AU - Toelen, Jaan

AU - Casteels, Cindy

AU - Macchi, Francesca

AU - Van Rompuy, Anne-Sophie

AU - Sarre, Sophie

AU - Casadei, Nicolas

AU - Nuber, Silke

AU - Himmelreich, Uwe

AU - Osorio Garcia, Maria Isabel

AU - Michotte, Yvette

AU - D'Hooge, Rudi

AU - Bormans, Guy

AU - Van Laere, Koen

AU - Gijsbers, Rik

AU - Van den Haute, Chris

AU - Debyser, Zeger

AU - Baekelandt, Veerle

PY - 2015

Y1 - 2015

N2 - Testing of new therapeutic strategies for Parkinson's disease (PD) is currently hampered by the lack of relevant and reproducible animal models. Here, we developed a robust rat model for PD by injection of adeno-associated viral vectors (rAAV2/7) encoding α-synuclein into the substantia nigra, resulting in reproducible nigrostriatal pathology and behavioral deficits in a 4-week time period. Progressive dopaminergic dysfunction was corroborated by histopathologic and biochemical analysis, motor behavior testing and in vivo microdialysis. L-DOPA treatment was found to reverse the behavioral phenotype. Non-invasive positron emission tomography imaging and magnetic resonance spectroscopy allowed longitudinal monitoring of neurodegeneration. In addition, insoluble α-synuclein aggregates were formed in this model. This α-synuclein rat model shows improved face and predictive validity, and therefore offers the possibility to reliably test novel therapeutics. Furthermore, it will be of great value for further research into the molecular pathogenesis of PD and the importance of α-synuclein aggregation in the disease process.

AB - Testing of new therapeutic strategies for Parkinson's disease (PD) is currently hampered by the lack of relevant and reproducible animal models. Here, we developed a robust rat model for PD by injection of adeno-associated viral vectors (rAAV2/7) encoding α-synuclein into the substantia nigra, resulting in reproducible nigrostriatal pathology and behavioral deficits in a 4-week time period. Progressive dopaminergic dysfunction was corroborated by histopathologic and biochemical analysis, motor behavior testing and in vivo microdialysis. L-DOPA treatment was found to reverse the behavioral phenotype. Non-invasive positron emission tomography imaging and magnetic resonance spectroscopy allowed longitudinal monitoring of neurodegeneration. In addition, insoluble α-synuclein aggregates were formed in this model. This α-synuclein rat model shows improved face and predictive validity, and therefore offers the possibility to reliably test novel therapeutics. Furthermore, it will be of great value for further research into the molecular pathogenesis of PD and the importance of α-synuclein aggregation in the disease process.

KW - Adeno-associated viral vectors

M3 - Article

VL - 36

SP - 1543

EP - 1558

JO - Neurobiology of Aging

JF - Neurobiology of Aging

SN - 0197-4580

ER -

ID: 5134949