DOI

Raman spectroscopy is a powerful tool for analytical measurements in many applications. Traditional Raman spectroscopic analyses require bulky equipment, considerable time of signal acquisition and manual sampling of substances under test. In this paper, we take a step from bulky and manual consuming laboratory testing towards lab-on-chip (LOC) analyses. We miniaturize the Raman spectroscopic system by combining a free-form reflector based polymer LOC with a customized Raman probe. By using the confocal detection principle, we aim to enhance the detection of the Raman signals from the substance of interest due to the suppression of the background Raman signal from the polymer of the chip. Next to the LOC we miniaturize the external optical components, surrounding the reflector embedding optofluidic chip, and assemble these in a Raman probe. We evaluate the misalignment tolerance of internal optics (LOC) and external optics (Raman probe) by non-sequential ray tracing which shows that off-axis misalignment is around +or-400mum and the maximum working distance of our Raman probe is 71mm. Using this probe, the system could be implemented as a portable reader unit containing the external optics, in which a low-cost, robust and mass manufacturable microfluidic LOC containing a freeform reflector is inserted, to enable confocal Raman spectroscopy measurements.
Original languageEnglish
Title of host publicationOptical System Alignment, Tolerancing, and Verification XI
EditorsJose Sasian, Richard N. Youngworth
PublisherSPIE
Number of pages9
Volume10377
ISBN (Electronic)9781510612112
ISBN (Print)978-1-5106-1211-2
DOIs
Publication statusPublished - 2017
EventOptical System Alignment, Tolerancing, and Verification XI - San Diego, California, United States
Duration: 6 Aug 20177 Aug 2017

Publication series

NameProceedings of SPIE

Conference

ConferenceOptical System Alignment, Tolerancing, and Verification XI
CountryUnited States
CityCalifornia
Period6/08/177/08/17

    Research areas

  • Raman spectroscopy, confocal detection, free-form, lab-on-chip, polymer, probe

ID: 36201013