Standard

Impact of particle size gradients on the apparent efficiency of chromatographic columns. / Codesido, Santiago; Rudaz, Serge; Veuthey, Jean-Luc; Guillarme, Davy; Desmet, Gert; Fekete, Szabolcs.

In: Journal of Chromatography A, Vol. 1603, 11.10.2019, p. 208-215.

Research output: Contribution to journalArticle

Harvard

Codesido, S, Rudaz, S, Veuthey, J-L, Guillarme, D, Desmet, G & Fekete, S 2019, 'Impact of particle size gradients on the apparent efficiency of chromatographic columns', Journal of Chromatography A, vol. 1603, pp. 208-215. https://doi.org/10.1016/j.chroma.2019.06.048

APA

Codesido, S., Rudaz, S., Veuthey, J-L., Guillarme, D., Desmet, G., & Fekete, S. (2019). Impact of particle size gradients on the apparent efficiency of chromatographic columns. Journal of Chromatography A, 1603, 208-215. https://doi.org/10.1016/j.chroma.2019.06.048

Vancouver

Codesido S, Rudaz S, Veuthey J-L, Guillarme D, Desmet G, Fekete S. Impact of particle size gradients on the apparent efficiency of chromatographic columns. Journal of Chromatography A. 2019 Oct 11;1603:208-215. https://doi.org/10.1016/j.chroma.2019.06.048

Author

Codesido, Santiago ; Rudaz, Serge ; Veuthey, Jean-Luc ; Guillarme, Davy ; Desmet, Gert ; Fekete, Szabolcs. / Impact of particle size gradients on the apparent efficiency of chromatographic columns. In: Journal of Chromatography A. 2019 ; Vol. 1603. pp. 208-215.

BibTeX

@article{2cda57e1eb624171957bf21c19b6a12a,
title = "Impact of particle size gradients on the apparent efficiency of chromatographic columns",
abstract = "In this paper, the benefits of using columns packed with particles of decreasing size (particle size gradient) in liquid chromatography was investigated from a theoretical point of view. It is indeed well known that such columns may be useful in gradient elution, since the decrease of particle size along the chromatographic column can provide extra peak focusing effect. In the present contribution, several parameters (i.e., mobile phase gradient steepness, retention times and operating pressures) were considered and the kinetic performance of various types of columns packed with particle size gradient were evaluated. In the best case, about 15–20{\%} gain in efficiency can be expected at a given retention time when utilizing a particle size gradient, compared to constant particle size. Conversely, when fixing efficiency, the analysis time can be decreased by about 15{\%} with an optimal particle size gradient. However, it is also important to keep in mind that a too large a particle size gradient can result in lower efficiencies than a column packed with monodisperse packing. We have introduced the g d value, which is a dimensionless measure of the particle size gradient steepness that measures the relative variation of particle diameter throughout the column with respect to the average. We finally observed that g d=0.3-0.4 provides the highest gain under practically useful conditions.",
keywords = "Particle size gradient, Apparent efficiency, Plate number, Gradient elution mode, Analysis time",
author = "Santiago Codesido and Serge Rudaz and Jean-Luc Veuthey and Davy Guillarme and Gert Desmet and Szabolcs Fekete",
year = "2019",
month = "10",
day = "11",
doi = "10.1016/j.chroma.2019.06.048",
language = "English",
volume = "1603",
pages = "208--215",
journal = "Journal of Chromatography. A",
issn = "0021-9673",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Impact of particle size gradients on the apparent efficiency of chromatographic columns

AU - Codesido, Santiago

AU - Rudaz, Serge

AU - Veuthey, Jean-Luc

AU - Guillarme, Davy

AU - Desmet, Gert

AU - Fekete, Szabolcs

PY - 2019/10/11

Y1 - 2019/10/11

N2 - In this paper, the benefits of using columns packed with particles of decreasing size (particle size gradient) in liquid chromatography was investigated from a theoretical point of view. It is indeed well known that such columns may be useful in gradient elution, since the decrease of particle size along the chromatographic column can provide extra peak focusing effect. In the present contribution, several parameters (i.e., mobile phase gradient steepness, retention times and operating pressures) were considered and the kinetic performance of various types of columns packed with particle size gradient were evaluated. In the best case, about 15–20% gain in efficiency can be expected at a given retention time when utilizing a particle size gradient, compared to constant particle size. Conversely, when fixing efficiency, the analysis time can be decreased by about 15% with an optimal particle size gradient. However, it is also important to keep in mind that a too large a particle size gradient can result in lower efficiencies than a column packed with monodisperse packing. We have introduced the g d value, which is a dimensionless measure of the particle size gradient steepness that measures the relative variation of particle diameter throughout the column with respect to the average. We finally observed that g d=0.3-0.4 provides the highest gain under practically useful conditions.

AB - In this paper, the benefits of using columns packed with particles of decreasing size (particle size gradient) in liquid chromatography was investigated from a theoretical point of view. It is indeed well known that such columns may be useful in gradient elution, since the decrease of particle size along the chromatographic column can provide extra peak focusing effect. In the present contribution, several parameters (i.e., mobile phase gradient steepness, retention times and operating pressures) were considered and the kinetic performance of various types of columns packed with particle size gradient were evaluated. In the best case, about 15–20% gain in efficiency can be expected at a given retention time when utilizing a particle size gradient, compared to constant particle size. Conversely, when fixing efficiency, the analysis time can be decreased by about 15% with an optimal particle size gradient. However, it is also important to keep in mind that a too large a particle size gradient can result in lower efficiencies than a column packed with monodisperse packing. We have introduced the g d value, which is a dimensionless measure of the particle size gradient steepness that measures the relative variation of particle diameter throughout the column with respect to the average. We finally observed that g d=0.3-0.4 provides the highest gain under practically useful conditions.

KW - Particle size gradient

KW - Apparent efficiency

KW - Plate number

KW - Gradient elution mode

KW - Analysis time

UR - http://www.scopus.com/inward/record.url?scp=85068051346&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2019.06.048

DO - 10.1016/j.chroma.2019.06.048

M3 - Article

VL - 1603

SP - 208

EP - 215

JO - Journal of Chromatography. A

JF - Journal of Chromatography. A

SN - 0021-9673

ER -

ID: 48665329