Liquid chromatography coupled with mass spectrometric detection is one of the major technologies used for protein sequencing, identification, and quantification. This review provides an introduction of the current state-of-the-art technology in peptide profiling using nano-liquid chromatography–mass spectrometry applied to large-scale protein or proteome analysis. In particular, different aspects of the bottom-up proteomics workflow are covered, including aspects of sample preparation such as protein digestion, nanoflow gradient reversed-phase chromatographic separation, LC–MS interfacing via electrospray ionization, tandem mass spectrometry of digests, protein identification via database searches, and finally peptide quantitation.
Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalChromatographia
Volume82
Issue number1
DOIs
Publication statusPublished - 7 Nov 2018

    Research areas

  • Nano-LC, Peptide mapping, Peptide profiling, Proteomics research, Review

ID: 44048809