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Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon. / Shintouo, Cabirou Mounchili; Nguve, Joel Ebai; Asa, Fru Bertha; Shey, Robert Adamu; Kamga, Joseph; Souopgui, Jacob; Ghogomu, Stephen Mbigha; Njemini, Rose.

In: PLoS Pathogens, Vol. 9, No. 9, E722, 02.09.2020.

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Harvard

Shintouo, CM, Nguve, JE, Asa, FB, Shey, RA, Kamga, J, Souopgui, J, Ghogomu, SM & Njemini, R 2020, 'Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon', PLoS Pathogens, vol. 9, no. 9, E722. https://doi.org/10.3390/pathogens9090722

APA

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Author

Shintouo, Cabirou Mounchili ; Nguve, Joel Ebai ; Asa, Fru Bertha ; Shey, Robert Adamu ; Kamga, Joseph ; Souopgui, Jacob ; Ghogomu, Stephen Mbigha ; Njemini, Rose. / Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon. In: PLoS Pathogens. 2020 ; Vol. 9, No. 9.

BibTeX

@article{97601f83f3a647948fceee28cd716c47,
title = "Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon",
abstract = "The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies-in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed-in each community-for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6{\%} in Makouopsap and 0.0{\%} in the other communities. On the other hand, the frequency of infected flies was 0.8{\%} in Makouopsap, 0.2{\%} in Bankambe, 0.1{\%} in Bayon, and 0.0{\%} in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities.",
keywords = "O-150 PCR, elimination, ivermectin, onchocerciasis, poolscreen",
author = "Shintouo, {Cabirou Mounchili} and Nguve, {Joel Ebai} and Asa, {Fru Bertha} and Shey, {Robert Adamu} and Joseph Kamga and Jacob Souopgui and Ghogomu, {Stephen Mbigha} and Rose Njemini",
year = "2020",
month = "9",
day = "2",
doi = "10.3390/pathogens9090722",
language = "English",
volume = "9",
journal = "PLoS Pathogens",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - Entomological Assessment of Onchocerca Species Transmission by Black Flies in Selected Communities in the West Region of Cameroon

AU - Shintouo, Cabirou Mounchili

AU - Nguve, Joel Ebai

AU - Asa, Fru Bertha

AU - Shey, Robert Adamu

AU - Kamga, Joseph

AU - Souopgui, Jacob

AU - Ghogomu, Stephen Mbigha

AU - Njemini, Rose

PY - 2020/9/2

Y1 - 2020/9/2

N2 - The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies-in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed-in each community-for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6% in Makouopsap and 0.0% in the other communities. On the other hand, the frequency of infected flies was 0.8% in Makouopsap, 0.2% in Bankambe, 0.1% in Bayon, and 0.0% in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities.

AB - The enormity of the public health burden of onchocerciasis motivated the creation of various large-scale control programs that have depended principally on mass treatment of endemic communities with ivermectin for the elimination of the disease. Parasitological evaluation of Onchocerca species in the West Region of Cameroon indicates significant progress in the interruption of parasite transmission in some communities under ivermectin treatment. However, to verify the complete elimination of onchocerciasis, entomological assessment through O-150 PCR poolscreen of black flies is mandatory. Thus, in the present study, we assessed transmission of Onchocerca species using an O-150 PCR technique to screen pools of black flies-in seven onchocerciasis endemic communities (Makouopsap, Bankambe, Lemgo, Tsesse, Ndionzou, Kouffen, and Bayon) in Cameroon. Two thousand black flies were assessed-in each community-for the presence of Onchocerca species DNA. Our results show that the frequency of infective flies was 0.6% in Makouopsap and 0.0% in the other communities. On the other hand, the frequency of infected flies was 0.8% in Makouopsap, 0.2% in Bankambe, 0.1% in Bayon, and 0.0% in Lemgo, Tsesse, Ndionzou, and Kouffen. These results provide entomologic evidence for continuous transmission of Onchocerca species in Makouopsap, risk of active transmission in Bankambe, and Bayon, and a suppressed transmission in the four other studied communities.

KW - O-150 PCR

KW - elimination

KW - ivermectin

KW - onchocerciasis

KW - poolscreen

U2 - 10.3390/pathogens9090722

DO - 10.3390/pathogens9090722

M3 - Article

C2 - 32887231

VL - 9

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 9

M1 - E722

ER -

ID: 53530574