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Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L. / Perez-Alonso, Naivy; Martin, Randel; Capote, Alina; Perez, Anabel; Hernandez-Diaz, Elizabeth Kairuz; Rojas, Luis; Jimenez, Elio; Quiala, Elisa; Angenon, Geert; Garcia-Gonzales, Rolando; Chong-Perez, Borys.

In: Industrial Crops and Products, Vol. 116, 06.2018, p. 259-266.

Research output: Contribution to journalArticle

Harvard

Perez-Alonso, N, Martin, R, Capote, A, Perez, A, Hernandez-Diaz, EK, Rojas, L, Jimenez, E, Quiala, E, Angenon, G, Garcia-Gonzales, R & Chong-Perez, B 2018, 'Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.', Industrial Crops and Products, vol. 116, pp. 259-266. https://doi.org/10.1016/j.indcrop.2018.02.067

APA

Perez-Alonso, N., Martin, R., Capote, A., Perez, A., Hernandez-Diaz, E. K., Rojas, L., ... Chong-Perez, B. (2018). Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L. Industrial Crops and Products, 116, 259-266. https://doi.org/10.1016/j.indcrop.2018.02.067

Vancouver

Perez-Alonso N, Martin R, Capote A, Perez A, Hernandez-Diaz EK, Rojas L et al. Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L. Industrial Crops and Products. 2018 Jun;116:259-266. https://doi.org/10.1016/j.indcrop.2018.02.067

Author

Perez-Alonso, Naivy ; Martin, Randel ; Capote, Alina ; Perez, Anabel ; Hernandez-Diaz, Elizabeth Kairuz ; Rojas, Luis ; Jimenez, Elio ; Quiala, Elisa ; Angenon, Geert ; Garcia-Gonzales, Rolando ; Chong-Perez, Borys. / Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L. In: Industrial Crops and Products. 2018 ; Vol. 116. pp. 259-266.

BibTeX

@article{0b1c3e54d3344e56b68d28beac673cab,
title = "Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.",
abstract = "Cardiovascular and cancer diseases are the first causes of death in the world. Digitalis purpurea L. is a medicinal plant that produces secondary metabolites, like digoxin and digitoxin, which are employed in therapies against heart failure. Moreover, anticancer and antiviral properties of these metabolites have recently been described. The present work details a method to obtain in vitro plants of D. purpurea from leaf segments through direct organogenesis. A reliable and efficient plant induction system was established by optimizing the concentration of naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BAP). The highest frequency of shoot regeneration (98.5{\%}) with an average number of shoots per leaf segment of 18.9 was achieved via direct organogenesis from leaf segment on MS medium containing 0.54 μM NAA + 13.2 μM 6-BAP. Additionally, Random Amplified Polymorphic DNA (RAPD) analysis showed 100{\%} monomorphic bands, which indicated genetic stability of the obtained plants. Moreover, leaf powder of de novo regenerated plants fulfills the quality specifications of the British Pharmacopoeia and HPLC analysis revealed the presence of digoxin (22.6 μg gDW −1) and digitoxin (220.7 μg gDW −1) without significant differences in contents between de novo regenerated and mother plants. An efficient in vitro propagation protocol via direct organogenesis and genetic stability assessment of D. purpurea for obtaining leaf powder with quality for the use as raw material have thus been described. The protocol also provides an effective means for several approaches in biotechnology and breeding programs, in order to produce pharmaceutically interesting cardenolides.",
keywords = "Cardenolides, Direct organogenesis, Foxglove, Genetic stability, Quality specification",
author = "Naivy Perez-Alonso and Randel Martin and Alina Capote and Anabel Perez and Hernandez-Diaz, {Elizabeth Kairuz} and Luis Rojas and Elio Jimenez and Elisa Quiala and Geert Angenon and Rolando Garcia-Gonzales and Borys Chong-Perez",
year = "2018",
month = "6",
doi = "10.1016/j.indcrop.2018.02.067",
language = "English",
volume = "116",
pages = "259--266",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Efficient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

AU - Perez-Alonso, Naivy

AU - Martin, Randel

AU - Capote, Alina

AU - Perez, Anabel

AU - Hernandez-Diaz, Elizabeth Kairuz

AU - Rojas, Luis

AU - Jimenez, Elio

AU - Quiala, Elisa

AU - Angenon, Geert

AU - Garcia-Gonzales, Rolando

AU - Chong-Perez, Borys

PY - 2018/6

Y1 - 2018/6

N2 - Cardiovascular and cancer diseases are the first causes of death in the world. Digitalis purpurea L. is a medicinal plant that produces secondary metabolites, like digoxin and digitoxin, which are employed in therapies against heart failure. Moreover, anticancer and antiviral properties of these metabolites have recently been described. The present work details a method to obtain in vitro plants of D. purpurea from leaf segments through direct organogenesis. A reliable and efficient plant induction system was established by optimizing the concentration of naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BAP). The highest frequency of shoot regeneration (98.5%) with an average number of shoots per leaf segment of 18.9 was achieved via direct organogenesis from leaf segment on MS medium containing 0.54 μM NAA + 13.2 μM 6-BAP. Additionally, Random Amplified Polymorphic DNA (RAPD) analysis showed 100% monomorphic bands, which indicated genetic stability of the obtained plants. Moreover, leaf powder of de novo regenerated plants fulfills the quality specifications of the British Pharmacopoeia and HPLC analysis revealed the presence of digoxin (22.6 μg gDW −1) and digitoxin (220.7 μg gDW −1) without significant differences in contents between de novo regenerated and mother plants. An efficient in vitro propagation protocol via direct organogenesis and genetic stability assessment of D. purpurea for obtaining leaf powder with quality for the use as raw material have thus been described. The protocol also provides an effective means for several approaches in biotechnology and breeding programs, in order to produce pharmaceutically interesting cardenolides.

AB - Cardiovascular and cancer diseases are the first causes of death in the world. Digitalis purpurea L. is a medicinal plant that produces secondary metabolites, like digoxin and digitoxin, which are employed in therapies against heart failure. Moreover, anticancer and antiviral properties of these metabolites have recently been described. The present work details a method to obtain in vitro plants of D. purpurea from leaf segments through direct organogenesis. A reliable and efficient plant induction system was established by optimizing the concentration of naphthaleneacetic acid (NAA) and 6-benzylaminopurine (6-BAP). The highest frequency of shoot regeneration (98.5%) with an average number of shoots per leaf segment of 18.9 was achieved via direct organogenesis from leaf segment on MS medium containing 0.54 μM NAA + 13.2 μM 6-BAP. Additionally, Random Amplified Polymorphic DNA (RAPD) analysis showed 100% monomorphic bands, which indicated genetic stability of the obtained plants. Moreover, leaf powder of de novo regenerated plants fulfills the quality specifications of the British Pharmacopoeia and HPLC analysis revealed the presence of digoxin (22.6 μg gDW −1) and digitoxin (220.7 μg gDW −1) without significant differences in contents between de novo regenerated and mother plants. An efficient in vitro propagation protocol via direct organogenesis and genetic stability assessment of D. purpurea for obtaining leaf powder with quality for the use as raw material have thus been described. The protocol also provides an effective means for several approaches in biotechnology and breeding programs, in order to produce pharmaceutically interesting cardenolides.

KW - Cardenolides

KW - Direct organogenesis

KW - Foxglove

KW - Genetic stability

KW - Quality specification

UR - http://www.scopus.com/inward/record.url?scp=85042847153&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2018.02.067

DO - 10.1016/j.indcrop.2018.02.067

M3 - Article

VL - 116

SP - 259

EP - 266

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

ER -

ID: 38829092