In the first part of this talk we will address the two-step mechanism of
nucleation of condensed phases. Our recent studies on the nucleation of protein
crystals and the characterization of the pre-nucleation phases will be presented. 1,2
Nucleation and formation of ordered and amorphous aggregates underlies a wide
range of human disorders including neurodegenerative and cardiovascular diseases 3 .
We will discuss the aggregation of the low complexity domain of the protein
hnRNPA2 involved in Amyotrophic lateral sclerosis (ALS). This intrinsically
disordered protein is prone to liquid-liquid phase separation and forms metastable
protein-rich phases that precede and facilitate the formation of aggregates.
In the second part we will elaborate on the effect of flow on crystallization.
Both theory 4 and experiments 5 have indicated that flow affects the nucleation rate, the
size of the emerging phase and polymorphism. To enable detailed quantitative
studies on the influence of shear flow on nucleation and growth, we have developed
a new microfluidic setup that creates a controlled constant shear flow profile in a
solution. Our experimental results will be complemented by kinetic Monte Carlo
simulations.
Original languageEnglish
Title of host publicationAbstract book
StateUnpublished - 16 Sep 2018
Event6th European Conference on Crystal Growth - Riviera Holiday Club, Varna, Bulgaria
Duration: 16 Sep 201820 Sep 2018
Conference number: 6

Conference

Conference6th European Conference on Crystal Growth
Abbreviated titleECCG
CountryBulgaria
CityVarna
Period16/09/1820/09/18

ID: 40179371