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Cholestasis differentially affects liver connexins. / Cooreman, Axelle; Van Campenhout, Raf; Crespo Yanguas, Sara; Gijbels, Eva; Leroy, Kaat; Pieters, Alanah; Tabernilla Garcia, Andres; Van Brantegem, Pieter; Annaert, Pieter; Cogliati, Bruno; Vinken, Mathieu.

In: International Journal of Molecular Sciences, Vol. 21, No. 18, 6534, 07.09.2020.

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Cooreman, Axelle ; Van Campenhout, Raf ; Crespo Yanguas, Sara ; Gijbels, Eva ; Leroy, Kaat ; Pieters, Alanah ; Tabernilla Garcia, Andres ; Van Brantegem, Pieter ; Annaert, Pieter ; Cogliati, Bruno ; Vinken, Mathieu. / Cholestasis differentially affects liver connexins. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 18.

BibTeX

@article{756d8795763f45d5bad81e25959e8e48,
title = "Cholestasis differentially affects liver connexins.",
abstract = "Connexins are goal keepers of tissue homeostasis, including in liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.",
keywords = "connexin, liver, cholestasis",
author = "Axelle Cooreman and {Van Campenhout}, Raf and {Crespo Yanguas}, Sara and Eva Gijbels and Kaat Leroy and Alanah Pieters and {Tabernilla Garcia}, Andres and {Van Brantegem}, Pieter and Pieter Annaert and Bruno Cogliati and Mathieu Vinken",
year = "2020",
month = "9",
day = "7",
doi = "10.3390/ijms21186534",
language = "English",
volume = "21",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "18",

}

RIS

TY - JOUR

T1 - Cholestasis differentially affects liver connexins.

AU - Cooreman, Axelle

AU - Van Campenhout, Raf

AU - Crespo Yanguas, Sara

AU - Gijbels, Eva

AU - Leroy, Kaat

AU - Pieters, Alanah

AU - Tabernilla Garcia, Andres

AU - Van Brantegem, Pieter

AU - Annaert, Pieter

AU - Cogliati, Bruno

AU - Vinken, Mathieu

PY - 2020/9/7

Y1 - 2020/9/7

N2 - Connexins are goal keepers of tissue homeostasis, including in liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.

AB - Connexins are goal keepers of tissue homeostasis, including in liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.

KW - connexin

KW - liver

KW - cholestasis

U2 - 10.3390/ijms21186534

DO - 10.3390/ijms21186534

M3 - Article

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 18

M1 - 6534

ER -

ID: 53494835