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@article{85011d6790474cd0959e359f0a575677,
title = "Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC-MS",
abstract = "In recent years, neuropeptides and their receptors have received an increased interest in neuropharmacological research. Although these molecules are considered relatively small compared with proteins, their in vivo quantification using microdialysis is more challenging than for small molecules. Low microdialysis recoveries, aspecific adsorption and the presence of various multiply charged precursor ions during ESI-MS/MS detection hampers the in vivo quantification of these low abundant biomolecules. Every step in the workflow, from sampling until analysis, has to be optimized to enable the sensitive analysis of these compounds in microdialysates.",
author = "{Van Wanseele}, Yannick and {De Prins}, An and {De Bundel}, Dimitri and Ilse Smolders and {Van Eeckhaut}, Ann",
year = "2016",
month = "9",
doi = "10.4155/bio-2016-0119",
language = "English",
volume = "8",
pages = "1965--1985",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "18",

}

RIS

TY - JOUR

T1 - Challenges for the in vivo quantification of brain neuropeptides using microdialysis sampling and LC-MS

AU - Van Wanseele, Yannick

AU - De Prins, An

AU - De Bundel, Dimitri

AU - Smolders, Ilse

AU - Van Eeckhaut, Ann

PY - 2016/9

Y1 - 2016/9

N2 - In recent years, neuropeptides and their receptors have received an increased interest in neuropharmacological research. Although these molecules are considered relatively small compared with proteins, their in vivo quantification using microdialysis is more challenging than for small molecules. Low microdialysis recoveries, aspecific adsorption and the presence of various multiply charged precursor ions during ESI-MS/MS detection hampers the in vivo quantification of these low abundant biomolecules. Every step in the workflow, from sampling until analysis, has to be optimized to enable the sensitive analysis of these compounds in microdialysates.

AB - In recent years, neuropeptides and their receptors have received an increased interest in neuropharmacological research. Although these molecules are considered relatively small compared with proteins, their in vivo quantification using microdialysis is more challenging than for small molecules. Low microdialysis recoveries, aspecific adsorption and the presence of various multiply charged precursor ions during ESI-MS/MS detection hampers the in vivo quantification of these low abundant biomolecules. Every step in the workflow, from sampling until analysis, has to be optimized to enable the sensitive analysis of these compounds in microdialysates.

U2 - 10.4155/bio-2016-0119

DO - 10.4155/bio-2016-0119

M3 - Article

C2 - 27554986

VL - 8

SP - 1965

EP - 1985

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 18

ER -

ID: 25932674