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C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice. / Sprimont, Lindsay; Janssen, Pauline; De Swert, Kathleen; Gilloteaux, Jacques; Bouchat, Joanna; Verbruggen, Lise; Bentea, Eduard Mihai; Massie, Ann; Nicaise, Charles.

Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018. 2018.

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

Harvard

Sprimont, L, Janssen, P, De Swert, K, Gilloteaux, J, Bouchat, J, Verbruggen, L, Bentea, EM, Massie, A & Nicaise, C 2018, C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice. in Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018. 11th FENS Forum of Neuroscience, Berlin, Germany, 7/07/18.

APA

Sprimont, L., Janssen, P., De Swert, K., Gilloteaux, J., Bouchat, J., Verbruggen, L., ... Nicaise, C. (2018). C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice. In Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018

Vancouver

Sprimont L, Janssen P, De Swert K, Gilloteaux J, Bouchat J, Verbruggen L et al. C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice. In Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018. 2018

Author

Sprimont, Lindsay ; Janssen, Pauline ; De Swert, Kathleen ; Gilloteaux, Jacques ; Bouchat, Joanna ; Verbruggen, Lise ; Bentea, Eduard Mihai ; Massie, Ann ; Nicaise, Charles. / C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice. Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018. 2018.

BibTeX

@inbook{d12206c4db5b4f96921064d255eea27c,
title = "C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice",
abstract = "xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. According to preliminary data, xCT/Slc7a11 mRNA is upregulated in the early phase following spinal cord injury (SCI) in mice. The aim of this study is to examine the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). While xCT+/+ and xCT-/- mice partly recovered motor functions, contused xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+cells was unexpectedly much higher in contused xCT-/- than in xCT+/+spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and compromises spinal motor neuron survival.",
author = "Lindsay Sprimont and Pauline Janssen and {De Swert}, Kathleen and Jacques Gilloteaux and Joanna Bouchat and Lise Verbruggen and Bentea, {Eduard Mihai} and Ann Massie and Charles Nicaise",
year = "2018",
language = "English",
booktitle = "Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018",

}

RIS

TY - CHAP

T1 - C113 - xCT/Slc7a11 deletion accelerates functional recovery and improves histological outcomes following cervical spinal contusion in mice

AU - Sprimont, Lindsay

AU - Janssen, Pauline

AU - De Swert, Kathleen

AU - Gilloteaux, Jacques

AU - Bouchat, Joanna

AU - Verbruggen, Lise

AU - Bentea, Eduard Mihai

AU - Massie, Ann

AU - Nicaise, Charles

PY - 2018

Y1 - 2018

N2 - xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. According to preliminary data, xCT/Slc7a11 mRNA is upregulated in the early phase following spinal cord injury (SCI) in mice. The aim of this study is to examine the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). While xCT+/+ and xCT-/- mice partly recovered motor functions, contused xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+cells was unexpectedly much higher in contused xCT-/- than in xCT+/+spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and compromises spinal motor neuron survival.

AB - xCT protein is the specific subunit of System xc-, a multi-proteic complex importing cystine into cell while releasing extracellular glutamate. Although xCT protein expression is detected in brain glial cells, its expression in the spinal cord and during spinal cord disorders remain elusive. According to preliminary data, xCT/Slc7a11 mRNA is upregulated in the early phase following spinal cord injury (SCI) in mice. The aim of this study is to examine the contribution of xCT to functional and histological outcomes following SCI, using wild-type mice (xCT+/+) and genetically-invalidated mice (xCT-/-). While xCT+/+ and xCT-/- mice partly recovered motor functions, contused xCT-/- mice recovered muscular grip strength as well as pre-SCI weight substantially faster than xCT+/+ mice. Histology of injured spinal cords revealed increased number of motor neurons in xCT-/- mice at multiple distances around lesion epicenter. As xCT has been demonstrated as a regulator of microglial function (Mesci et al., Brain, 2015), we assessed markers of microglial activation. At 2 weeks post-SCI, the number of type A Iba1+cells was unexpectedly much higher in contused xCT-/- than in xCT+/+spinal cords. Analysis of M1/M2 polarization showed that contused xCT-/- spinal cords contained higher mRNA levels of Ym1 and IGF-1 (M2) while lower levels of NOX2 and TNF-a (M1). Additionally, the number of astrocytes and oligodendrocytes were unchanged between the two injured groups. This study suggests that, following SCI trauma, an early xCT upregulation (if confirmed at the protein level) exacerbates microglia-driven inflammation and compromises spinal motor neuron survival.

UR - https://ep70.eventpilot.us/web/page.php?page=Session&project=FENS18&id=P_0000004390_0

M3 - Meeting abstract (Book)

BT - Federation of European Neuroscience Society, 11th FENS Forum of Neuroscience, Berlin, 7-11 july, 2018

ER -

ID: 44031034