Standard

Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse. / Segers, Ingrid; Adriaenssens, Tom; Ozturk, Elife; Smitz, Johan.

In: Fertility and Sterility, Vol. 93, 2010, p. 2695-2700.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

BibTeX

@article{5ed4ec6a43ee4bb19da74403ad7637e5,
title = "Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse.",
abstract = "OBJECTIVE: To analyze the effect of various culture periods and FSH on oocyte competence in cultured mouse follicles. DESIGN: Controlled in vitro laboratory experiment. SETTING: Academic research environment. INTERVENTION(S): DNA staining, maturation induction, parthenogenesis, and IVF were performed after various periods of oocyte culture. Additionally, FSH modulation during preantral follicle growth was studied. MAIN OUTCOME MEASURE(S): Nucleolar conformation, meiotic resumption, activation potential, and preimplantation development were recorded. RESULT(S): Surrounded nucleolus (SN) conformation was found in oocytes cultured for 8 days and correlated by time with meiotic competence. Culture for 12 days increased oocyte diameter (day 8 69.8 mum vs. day 12 72.4 mum) and meiotic competence (day 8 63{\%} vs. day 12 81{\%} polar body oocytes), but decreased fertilization rate (day 8 75{\%} vs. day 12 53{\%} 2-cell embryos). Equal activation potential (49{\%}) on both days indicated that impaired sperm entry could not account for lower fertilization rate after 12 days. Depriving early follicular growth of FSH delayed antrum formation but not SN conformation. CONCLUSION(S): Fertilization of cultured oocytes is most efficient close to the completion of SN. Omitting FSH from early follicle growth could not postpone the early onset of SN, which seems to be imposed by the time spent in vitro.",
keywords = "follicle culture, IVF, oocyte maturation, nucleolar conformation, FSH",
author = "Ingrid Segers and Tom Adriaenssens and Elife Ozturk and Johan Smitz",
year = "2010",
language = "English",
volume = "93",
pages = "2695--2700",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse.

AU - Segers, Ingrid

AU - Adriaenssens, Tom

AU - Ozturk, Elife

AU - Smitz, Johan

PY - 2010

Y1 - 2010

N2 - OBJECTIVE: To analyze the effect of various culture periods and FSH on oocyte competence in cultured mouse follicles. DESIGN: Controlled in vitro laboratory experiment. SETTING: Academic research environment. INTERVENTION(S): DNA staining, maturation induction, parthenogenesis, and IVF were performed after various periods of oocyte culture. Additionally, FSH modulation during preantral follicle growth was studied. MAIN OUTCOME MEASURE(S): Nucleolar conformation, meiotic resumption, activation potential, and preimplantation development were recorded. RESULT(S): Surrounded nucleolus (SN) conformation was found in oocytes cultured for 8 days and correlated by time with meiotic competence. Culture for 12 days increased oocyte diameter (day 8 69.8 mum vs. day 12 72.4 mum) and meiotic competence (day 8 63% vs. day 12 81% polar body oocytes), but decreased fertilization rate (day 8 75% vs. day 12 53% 2-cell embryos). Equal activation potential (49%) on both days indicated that impaired sperm entry could not account for lower fertilization rate after 12 days. Depriving early follicular growth of FSH delayed antrum formation but not SN conformation. CONCLUSION(S): Fertilization of cultured oocytes is most efficient close to the completion of SN. Omitting FSH from early follicle growth could not postpone the early onset of SN, which seems to be imposed by the time spent in vitro.

AB - OBJECTIVE: To analyze the effect of various culture periods and FSH on oocyte competence in cultured mouse follicles. DESIGN: Controlled in vitro laboratory experiment. SETTING: Academic research environment. INTERVENTION(S): DNA staining, maturation induction, parthenogenesis, and IVF were performed after various periods of oocyte culture. Additionally, FSH modulation during preantral follicle growth was studied. MAIN OUTCOME MEASURE(S): Nucleolar conformation, meiotic resumption, activation potential, and preimplantation development were recorded. RESULT(S): Surrounded nucleolus (SN) conformation was found in oocytes cultured for 8 days and correlated by time with meiotic competence. Culture for 12 days increased oocyte diameter (day 8 69.8 mum vs. day 12 72.4 mum) and meiotic competence (day 8 63% vs. day 12 81% polar body oocytes), but decreased fertilization rate (day 8 75% vs. day 12 53% 2-cell embryos). Equal activation potential (49%) on both days indicated that impaired sperm entry could not account for lower fertilization rate after 12 days. Depriving early follicular growth of FSH delayed antrum formation but not SN conformation. CONCLUSION(S): Fertilization of cultured oocytes is most efficient close to the completion of SN. Omitting FSH from early follicle growth could not postpone the early onset of SN, which seems to be imposed by the time spent in vitro.

KW - follicle culture

KW - IVF

KW - oocyte maturation

KW - nucleolar conformation

KW - FSH

M3 - Article

VL - 93

SP - 2695

EP - 2700

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

ER -

ID: 1884045