Objective: To compare the differential effects of HP-HMG or rFSH on cytokine expression before and after ovulation in an in vitro mouse ovarian follicle model.
Design: A prospective laboratory in vitro study
Setting: A university-based reproductive biology laboratory
Materials: Mechanically isolated mouse preantral follicles from 14 day old prepuberal mouse ovaries (F1 hybrids: C57BL/6JxCBA/ca)
Interventions: Randomly distributed mouse early preantral follicles were exposed to 2 hyperstimulation conditions with either HP-hMG or rFSH. An ovulatory stimulus was given using hCG/EGF. Conditioned media from the 2 culture conditions were collected on the days before and after in vitro ovulation. Conditioned media were compared for their relative cytokine profile content measured by a cytokine antibody array analysis.
Main Outcome Measures: Relative concentrations of 62 cytokines in conditioned media before and after ovulation.
Results: Statistically significant increase in the production of a number of cytokines was found after HP-hMG stimulation compared to rFSH: 14 and 24 pre- and post-rhCG, respectively. Cytokines with the largest significant difference (more than 5 times) before and after ovulation included TECK, sTNFRI and SDF-1. Cytokines most strongly related to oocyte and embryo quality and implantation, and that have been related to oocyte yield and maturation, were significantly higher with HP-hMG.

Conclusions: The significant differences in follicular cytokine production induced by HP-HMG and rFSH before and after in vitro ovulation might contribute to the difference in treatment outcome.
Original languageEnglish
Pages (from-to)1464-1476
Number of pages13
JournalFertility and Sterility
Volume93
Publication statusPublished - 2010

    Research areas

  • cytokines, HP-HMG, rFSH, oocyte, implantation, ovulation

ID: 1792073