Nanoparticles are classified as nano-objects having external dimensions between 1 and 100 nm. Their use as
basic building blocks or as autonomous functional entities is rapidly increasing along with the development of
nanobiotechnology, nanoelectronics and nanomedicine. It is the desire of industry, traders, consumers and
regulators to be able to classify nanoparticles according to their shape, size and surface properties. These
days, several sizing techniques for the characterisation of nanoparticles exist. However, differences between
measurement results are unfortunately no exception. This is due to an inadequate definition of the
measurands, the lack of standardised methods and a limited availability certified reference materials. The
objective of the project is to carefully select, based on their biochemical properties and particle size, different
pure proteins (around 10 nm) to prepare solutions or colloidal suspensions and to investigate the relationship
between changing environmental conditions and particle size by means of modulated temperature differential
scanning calorimetry, atomic force microscopy, gel electrophoresis and different particle sizing techniques
(dynamic light scattering, field-flow-fractionation, small angle X-ray scattering ...). This must lead, for the
selected model systems, to an improved understanding of the correlation between structure and functionality
on the one hand, and their particle size and shape on the other hand. Such well-characterised model
systems will enhance the efficiency and relevance of existing and newly to be developed particle
characterisation techniques.
Effective start/end date1/01/0931/12/09

    Flemish discipline codes

  • Chemical sciences

    Research areas

  • Chemistry

ID: 3273199